©2019 by Doran Bennett

Photosystem II Light Harvesting: Literature

Excitation Energy Transfer and Charge Separation in Photosystem II

Membranes Revisited

Koen Broess, Gediminas Trinkunas, Chantal D. van der Weij-de Wit, Jan P. Dekker, Arie van Hoek, and Herbert van Amerongen

Question 1: What is a BBY preparation? How are the 'lifetime' values the authors discuss relate to TCSPC measurements and what do they tell us about the process of light harvesting in the sample?

Question 2: What model do the authors use to simulate their fluorescence lifetimes? Try to understand both the physical meaning/interpretation of their model as well as how they represent it mathematically.

Question 3: What is the relative timescale of charge separation vs exciton migration in both their samples and PSII membranes more broadly?

Question 4: To what extent is their modeling approach "bottom-up" vs "top-down" and what does that mean for how we should understand their results?

A Structure-Based Model of Energy Transfer Reveals the Principles of

Light Harvesting in Photosystem II Supercomplexes

Doran I. G. Bennett, Kapil Amarnath, and Graham R. Fleming

Question 1: What is a photosystem II super complex and what is their role in light harvesting in vivo? What structural model was used for the simulations and how was it obtained? 

 

Question 2: What model is used for the simulations and how were the parameters determined? What is the physical meaning of 'domains of chlorophyll' and why are they convenient for calculations? 

 

Question 3: How were the time scales for diffusion, transfer-to-trap, and charge separation determined within the simulations? What does this suggest about the process of light harvesting both in isolated PSII super complexes and in vivo? 

Question 4: The authors note that the quality of fit cannot be used as a proxy for the quality of the excitation energy transfer model. How should we determine the quality of their results? To what extent are the presented simulations representative (or not) of the actual process of light harvesting in photosystem II? 

Multiscale model of light harvesting by photosystem II
in plants

Kapil Amarnatha, Doran I. G. Bennett, Anna R. Schneider, and Graham R. Fleming

Question 1: What model is used for the presented simulations? Pay attention to both the structural and excitation-energy transport components of the model. 

 

Question 2: What does it mean for excitation energy transport to be diffusive vs sub-diffusive? What is the excitation diffusion length and what does it mean for the process of light harvesting in the simulations?

 

Question 3: How does the formation of PSII-S crystalline arrays influence light-harvesting in the simulations? How do the authors interpret this result and how do you interpret it? (Meaning, what do you think it tells us about the real process of light harvesting in a PSII membrane?)

 

Question 4: What are the lake/puddle models and how have they been used previously? What do the current results suggest about the applicability of these models to PSII?

Energy-dependent quenching adjusts the excitation diffusion length to regulate photosynthetic light harvesting

Doran I. G. Bennett, Graham R. Fleming, and Kapil Amarnath

Question 1: What is NPQ and qE? How are they related to protecting photosystem II from bright light? 

 

Question 2: How is qE modeled in these simulations and what does it mean for the diffusional picture of excitation energy transfer? How was this model parameterized? 

 

Question 3: How does qE interact with PSII light harvesting and how is this reflected in the RC antenna size? 

 

Question 4: What is PAM fluorescence and how does it probe qE dynamics? Why are the 'lake' and 'puddle' models problematic according to the authors and what solution do they suggest? 

The Natural History of Model Organisms: From molecular manipulation of domesticated Chlamydomonas reinhardtii to survival in nature

Severin Sasso, Herwig Stibor, Maria Mittag and Arthur R Grossman

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Dynamic regulation of photosynthesis in Chlamydomonas reinhardtii

Jun Minagawa and Ryutaro Tokutsu

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Charting the native architecture of thylakoid membranes with single-molecule precision

Wojciech Wietrzynski, Miroslava Schaffer, Dimitry Tegunov, Sahradha Albert, Atsuko Kanazawa, Jürgen M. Plitzko, Wolfgang Baumeister, Benjamin D. Engel

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pH dependence, kinetics and light-harvesting regulation of nonphotochemical quenching

in Chlamydomonas

Lijin Tiana, Wojciech J. Nawrockia, Xin Liua, Iryna Polukhinaa, Ivo H. M. van Stokkuma, and Roberta Crocea

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Regulation and Dynamics of the Light-Harvesting System

Jean-David Rochaix

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Introduction to Chlamy: Literature